5V6X

Crystal structure of the tRNA binding domain of Pyrrolysyl-tRNA synthetase mutant (32A NTD) bound to tRNA(Pyl)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	72.620, 72.620, 238.580
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.330 - 2.760
R-factor	0.2107
Rwork	0.206
R-free	0.24900
RMSD bond length	0.003
RMSD bond angle	0.636
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.330
High resolution limit [Å]	2.760
Number of reflections	19670
<I/σ(I)>	25.7
Completeness [%]	99.9
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	Bis-Tris, MgCl2, PEG3350