5V6N

Crystal Structure of the highly open channel-stabilized mutant C27S + K33C + I9'A + N21'C of GLIC under reducing conditions.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-28
Detector	MAR CCD 165 mm
Wavelength(s)	0.97857
Spacegroup name	C 1 2 1
Unit cell lengths	177.178, 134.063, 159.680
Unit cell angles	90.00, 101.13, 90.00

Refinement procedure

Resolution	59.760 - 3.355
R-factor	0.2336
Rwork	0.231
R-free	0.28360
RMSD bond length	0.012
RMSD bond angle	1.627
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.163	3.367
High resolution limit [Å]	3.355	3.355
Rmerge	0.244	1.433
Rmeas	0.262	1.535
Rpim	0.095	0.549
Total number of observations	402323
Number of reflections	52515
<I/σ(I)>	9.6
Completeness [%]	99.9	100
Redundancy	7.7	7.8
CC(1/2)	0.979	0.743

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	200-250 mM (NH4)2SO4, 10-12% (wt/vol) PEG-4000 50 mM sodium acetate, pH 3.9-4.2