5V5V
Complex of NLGN2 with MDGA1 Ig1-Ig2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2016-03-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 |
| Unit cell lengths | 103.118, 97.182, 190.510 |
| Unit cell angles | 95.52, 80.97, 88.71 |
Refinement procedure
| Resolution | 49.770 - 4.110 |
| R-factor | 0.29032 |
| Rwork | 0.289 |
| R-free | 0.31861 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bl8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.550 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.770 | 4.180 |
| High resolution limit [Å] | 4.110 | 4.110 |
| Rmerge | 0.064 | 0.586 |
| Number of reflections | 53553 | 2112 |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 94.4 | 76.1 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.03 M NaNO3, 0.03 M Na2HPO4, 0.03 M (NH4)2SO4, 0.05 M HEPES pH 7.5, 0.05 M MOPS pH 7.5, 12 % (v/v) ethylene glycol, 9 % (w/v) PEG 8000 |
