5V5T
Crystal structure of leucine-rich protein regulator, ElrR, from Enterococcus faecalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-09-18 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 70.800, 75.330, 85.280 |
Unit cell angles | 112.99, 89.93, 103.88 |
Refinement procedure
Resolution | 36.550 - 2.150 |
R-factor | 0.1992 |
Rwork | 0.196 |
R-free | 0.24160 |
Structure solution method | SAD, MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 36.550 | 36.550 | 2.270 |
High resolution limit [Å] | 2.150 | 6.800 | 2.150 |
Rmerge | 0.024 | 0.542 | |
Rmeas | 0.062 | 0.031 | 0.705 |
Rpim | 0.039 | 0.019 | 0.447 |
Total number of observations | 185116 | 6345 | 17917 |
Number of reflections | 76473 | ||
<I/σ(I)> | 9.3 | 25.2 | 1.6 |
Completeness [%] | 89.8 | 97.4 | 60.7 |
Redundancy | 2.4 | 2.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | PEG 1000, PEG 3350, MPD, MOPS/HEPES-Na, L-glutamate, DL-alanine, glycine, DL-lysine, DL-serine |