5V5B

KVQIINKKLD, Structure of the amyloid spine from microtubule associated protein tau Repeat 2

Experimental procedure

Source type	ELECTRON MICROSCOPE
Source details	TECNAI F20 TEM
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-08-25
Detector	TVIPS TEMCAM-F416
Wavelength(s)	0.0251
Spacegroup name	P 1 21 1
Unit cell lengths	27.120, 4.830, 32.430
Unit cell angles	90.00, 100.53, 90.00

Refinement procedure

Resolution	18.830 - 1.500
R-factor	0.1927
Rwork	0.190
R-free	0.21270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.510
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	18.830	18.830	1.560
High resolution limit [Å]	1.500	5.410	1.500
Rmerge	0.255	0.166	0.419
Rmeas	0.268	0.177	0.452
Total number of observations	20119
Number of reflections	2203	28	281
<I/σ(I)>	5.24	8.4	2.83
Completeness [%]	84.8	62.2	85.9
Redundancy	9.133	8.893	6.214
CC(1/2)	0.984	0.979	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	310	0.1 M phosphate citrate, 32% PEG10000