5V4V

Saccharomyces cerevisiae Old Yellow Enzyme 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	298
Detector technology	CCD
Collection date	2015-10-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9787
Spacegroup name	P 21 21 21
Unit cell lengths	61.214, 107.762, 141.071
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.879 - 1.798
R-factor	0.1496
Rwork	0.147
R-free	0.18910
RMSD bond length	0.009
RMSD bond angle	1.245
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.880
High resolution limit [Å]	1.798
Number of reflections	87273
<I/σ(I)>	20.2
Completeness [%]	99.8
Redundancy	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	50 mM MES monohydrate, 22% v/v PEG 400, 25 mM Tris, 25 mM NaCl, pH 8.0