5V4T
Protein 84 with aldehyde deformylating oxygenase activity from Sulfolobus tokodaii (orthorhombic form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 71.466, 114.360, 64.857 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.120 - 1.700 |
| R-factor | 0.18978 |
| Rwork | 0.189 |
| R-free | 0.21963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uxg |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.726 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.120 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 59271 |
| <I/σ(I)> | 18.5 |
| Completeness [%] | 99.7 |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 10% ethanol, 1.5 M NaCl |
