5V4K

Crystal structure of NEDD4 LIR-fused human LC3B_2-119

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	69.570, 69.570, 119.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.496 - 2.099
R-factor	0.1845
Rwork	0.182
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.749
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.500
High resolution limit [Å]	2.100
Number of reflections	17887
<I/σ(I)>	18.4
Completeness [%]	100.0
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	0.1M Sodium Acetate pH 5.0, 1.2M Ammonium Sulfate