5V4E
Engineered human IgG Fc domain glyco801 (Fc801)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97648 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.615, 141.020, 98.866 |
| Unit cell angles | 90.00, 117.33, 90.00 |
Refinement procedure
| Resolution | 39.886 - 3.216 |
| R-factor | 0.2384 |
| Rwork | 0.236 |
| R-free | 0.28090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.614 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.280 |
| High resolution limit [Å] | 3.220 | 3.220 |
| Rmerge | 0.150 | |
| Number of reflections | 36958 | |
| <I/σ(I)> | 9.692 | 1.55 |
| Completeness [%] | 99.8 | |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 40 mM potassium phosphate 16% w/v PEG 8000 20% v/v glycerol |
