5V4A

A New Glycosyltransferase (DUF1792) from Streptococcus sanguinis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-16
Detector	RAYONIX MX300-HS
Wavelength(s)	1.00000
Spacegroup name	P 2 21 21
Unit cell lengths	42.650, 99.860, 140.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.300 - 1.750
R-factor	0.2086
Rwork	0.206
R-free	0.26850
RMSD bond length	0.007
RMSD bond angle	0.943
Data scaling software	XDS
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.310
High resolution limit [Å]	1.750
Number of reflections	60928
<I/σ(I)>	9.7
Completeness [%]	94.3
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	2M Ammonium Sulfate, 2% PEG 400, 0.1M HEPES pH 7.5