5V45
Crystal structure of the F270M, K291M, L318M mutant of SR1 domain of human sacsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.679, 41.360, 74.106 |
| Unit cell angles | 99.55, 95.26, 107.50 |
Refinement procedure
| Resolution | 50.000 - 1.910 |
| R-factor | 0.18826 |
| Rwork | 0.186 |
| R-free | 0.22404 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.384 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 32566 | 2240 |
| <I/σ(I)> | 43.5 | 4.5 |
| Completeness [%] | 96.2 | 89.1 |
| Redundancy | 7.9 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES pH 6.5, 28% PEG 3350, 0.2 M ammonium acetate |
