5V3M

mouseZFP568-ZnF1-11 in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-13
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	35.954, 92.758, 353.667
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.245 - 2.091
R-factor	0.2148
Rwork	0.213
R-free	0.25320
Structure solution method	SAD
RMSD bond length	0.025
RMSD bond angle	1.095
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.245	2.160
High resolution limit [Å]	2.090	2.090
Rmerge	0.067	0.479
Number of reflections	53292	1160
<I/σ(I)>	26	2.6
Completeness [%]	81.7	32.9
Redundancy	13.9	6.8
CC(1/2)		0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	10% Isopropanol, 0.1M Bicine(pH 8.5) and 30% (W/V) PEG1500