5V3M
mouseZFP568-ZnF1-11 in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-13 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 35.954, 92.758, 353.667 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.245 - 2.091 |
| R-factor | 0.2148 |
| Rwork | 0.213 |
| R-free | 0.25320 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.095 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.245 | 2.160 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.067 | 0.479 |
| Number of reflections | 53292 | 1160 |
| <I/σ(I)> | 26 | 2.6 |
| Completeness [%] | 81.7 | 32.9 |
| Redundancy | 13.9 | 6.8 |
| CC(1/2) | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 10% Isopropanol, 0.1M Bicine(pH 8.5) and 30% (W/V) PEG1500 |
