5V37
Crystal structure of SMYD3 with SAM and EPZ028862
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.818, 65.868, 107.015 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.260 - 1.420 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.27890 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.359 |
| Data reduction software | KYLIN |
| Data scaling software | KYLIN |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.260 | 1.450 |
| High resolution limit [Å] | 1.410 | 1.420 |
| Rmerge | 0.074 | 0.346 |
| Number of reflections | 75322 | |
| <I/σ(I)> | 8.8 | 2.6 |
| Completeness [%] | 97.7 | 85.9 |
| Redundancy | 5.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.10 M Magnesium formate, 0.1M Tris 8.0, 14% w/v PEG 3350 |
