5V2P
CaV beta2a subunit: CaV1.2 AID-CAP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2012-11-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.11587 |
| Spacegroup name | H 3 |
| Unit cell lengths | 81.805, 81.805, 163.271 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.18676 |
| Rwork | 0.184 |
| R-free | 0.23021 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t3s |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.193 |
| Data reduction software | iMOSFLM (7.0.4) |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.500 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.109 | 1.937 |
| Number of reflections | 23913 | |
| <I/σ(I)> | 5.9 | 0.4 |
| Completeness [%] | 86.6 | 90.1 |
| Redundancy | 1.3 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 277 | 34-37% PEG400, 0.1 M magnesium chloride, 0.1 M MES, pH 6.3 |
