5V2I
Crystal structure of a mutant glycosylasparaginase (G172D) that causes the genetic disease Aspartylglucosaminuria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.040, 52.770, 61.890 |
| Unit cell angles | 81.51, 90.15, 105.93 |
Refinement procedure
| Resolution | 20.000 - 1.830 |
| R-factor | 0.1577 |
| Rwork | 0.156 |
| R-free | 0.19667 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p4k |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.915 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.150 | 1.870 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 45208 | |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 92.6 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.2M Ammonium Acetate, 0.1M Bis-Tris pH 5.5, 25% Polyethylene glycol 3350 |
