5V1Z

Crystal structure of the RPN13 PRU-RPN2 (932-953)-ubiquitin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.127
Spacegroup name	P 31
Unit cell lengths	100.852, 100.852, 37.571
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.012 - 2.000
R-factor	0.1552
Rwork	0.146
R-free	0.18170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 2R2Y & 1CMX
RMSD bond length	0.009
RMSD bond angle	0.887
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	28875	2748
<I/σ(I)>	16	2.5
Completeness [%]	100.0	100
Redundancy	4.7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.1 M sodium acetate, pH 4.6, 22.5% PEG3350