5V1Y

Crystal structure of the ternary RPN13 PRU-RPN2 (940-953)-ubiquitin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 1 21 1
Unit cell lengths	36.129, 96.098, 57.605
Unit cell angles	90.00, 96.38, 90.00

Refinement procedure

Resolution	36.804 - 1.421
R-factor	0.1413
Rwork	0.140
R-free	0.17480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 2R2Y & 1CMX
RMSD bond length	0.008
RMSD bond angle	0.984
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.470
High resolution limit [Å]	1.420	1.420
Number of reflections	72405	2526
<I/σ(I)>	24.8	5.2
Completeness [%]	98.0	93
Redundancy	7.6	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	277	0.1 M citric acid, pH 4.6, 20% PEG6000