5V1K
Structure of R-GNA dodecamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-02-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.916978 |
| Spacegroup name | P 1 |
| Unit cell lengths | 23.681, 27.680, 29.050 |
| Unit cell angles | 73.00, 87.17, 66.58 |
Refinement procedure
| Resolution | 50.000 - 1.180 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.220 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Rmerge | 0.052 | 0.449 |
| Number of reflections | 20171 | 1914 |
| <I/σ(I)> | 37.5 | |
| Completeness [%] | 94.9 | 90.3 |
| Redundancy | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | Oligonucleotide (0.6 mM), sodium cacodylate (20 mM, pH 6.0), potassium chloride (40 mM), spermine tetrahydrochloride (6 mM), and 2-methyl-2,4-pentanediol (MPD; 5% (v/v) |
