5UZN

Crystal structure of Glorund qRRM3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-04
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54
Spacegroup name	P 42 21 2
Unit cell lengths	55.474, 55.474, 58.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.809 - 1.990
R-factor	0.2213
Rwork	0.220
R-free	0.25990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2kg1
RMSD bond length	0.010
RMSD bond angle	1.229
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.990
Rmerge	0.062
Number of reflections	6462
<I/σ(I)>	32.4
Completeness [%]	95.9
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.5 M ammonium sulfate, 0.1 M Na cacodylate pH 6.4, 1.0 M lithium sulfate