5UZE
Crystal Structure of Inosine 5'-monophosphate Dehydrogenase from Clostridium perfringens Complexed with IMP and P182
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.777, 77.662, 80.321 |
| Unit cell angles | 110.03, 103.99, 105.46 |
Refinement procedure
| Resolution | 31.387 - 2.270 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.224 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.310 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Number of reflections | 60049 | 2992 |
| <I/σ(I)> | 8.9 | 2.03 |
| Completeness [%] | 97.3 | 97 |
| Redundancy | 2.1 | 2.1 |
| CC(1/2) | 0.784 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 289 | 20% PEG600, 50mM ADA pH 6.8 |
