5UYJ
Crystal Structure of the Human CAMKK2B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976250 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.153, 73.153, 120.305 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.610 - 1.600 |
| R-factor | 0.154 |
| Rwork | 0.153 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zv2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.610 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.122 | 0.315 |
| Rpim | 0.025 | 0.063 |
| Number of reflections | 43864 | 2137 |
| <I/σ(I)> | 21.3 | 10.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 25.8 | |
| CC(1/2) | 0.997 | 0.989 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% PEG 3350, 20 mM Ammonium Sulphate, 0.1M Citric acid-Hepes-CHES buffer system |
