5UY6

Crystal Structure of the Human CAMKK2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979200
Spacegroup name	P 43 21 2
Unit cell lengths	73.324, 73.324, 121.964
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.750 - 1.700
R-factor	0.161
Rwork	0.160
R-free	0.18200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zv2
RMSD bond length	0.010
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.750	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.147	1.837
Rpim	0.032	0.398
Number of reflections	37374	1939
<I/σ(I)>	14.2	2
Completeness [%]	99.9	100
Redundancy	21.7	22.1
CC(1/2)	0.998	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	25% PEG 3350; 0.1M Bis-Tris; 0.2M ammonium acetate