5UXQ

Structure of anti-HIV trimer apex antibody PGT143

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	70.866, 97.643, 126.206
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.822 - 2.415
R-factor	0.2314
Rwork	0.229
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.900
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.830
High resolution limit [Å]	2.400
Number of reflections	32477
<I/σ(I)>	9.6
Completeness [%]	95.1
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	65% (v/v) 2-methyl-2,4-pentanediol and 0.1 M Tris, pH 8.0