5UXA
Crystal structure of macrolide 2'-phosphotransferase MphB from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 67.276, 116.684, 92.574 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.171 - 1.950 |
R-factor | 0.155 |
Rwork | 0.151 |
R-free | 0.20130 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.156 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_2481) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rpim | 0.033 | 0.282 |
Number of reflections | 27167 | 1354 |
<I/σ(I)> | 24.94 | 3.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.6 | 8.7 |
CC(1/2) | 0.905 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2 M calcium acetate, 0.1 M sodium cacodylate pH 6.5, 18% PEG 8K |