5UWW

Crystal Structure of DEAF1 Peptide in complex with CRM1 K579A mutant-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-02-19
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	106.043, 106.043, 304.207
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.241 - 2.152
R-factor	0.1865
Rwork	0.186
R-free	0.22600
RMSD bond length	0.002
RMSD bond angle	0.515
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.190
High resolution limit [Å]	2.150	2.150
Rmerge	0.090
Rpim	0.025	0.543
Number of reflections	95096	4653
<I/σ(I)>	32.2	1.5
Completeness [%]	100.0	100
Redundancy	13.7	13.8
CC(1/2)		0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	295	16% PEG3350, 100 mM Bis-Tris, pH 6.4, 200 mM ammonium nitrate, 16 mM HCl