5UWT

Crystal Structure of Hxk2 Peptide in complex with CRM1 K579A mutant-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2015-12-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	106.712, 106.712, 304.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.723 - 2.342
R-factor	0.1881
Rwork	0.187
R-free	0.22690
RMSD bond length	0.003
RMSD bond angle	0.468
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.340	2.340
Rmerge	0.105
Rpim	0.042	0.519
Number of reflections	74959	3673
<I/σ(I)>	18.9	1.4
Completeness [%]	100.0	100
Redundancy	7.2	7.2
CC(1/2)		0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	295	17% PEG3350, 100 mM Bis-Tris, pH 6.4, 200 mM ammonium nitrate, 20 mM HCl