5UWS

Crystal Structure of X11L2 NES Peptide in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2014-12-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	106.646, 106.646, 304.628
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.694 - 2.401
R-factor	0.1894
Rwork	0.188
R-free	0.22240
RMSD bond length	0.003
RMSD bond angle	0.479
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.094
Rpim	0.041	0.605
Number of reflections	69614	3419
<I/σ(I)>	24.4	1.7
Completeness [%]	99.9	100
Redundancy	9.4	7.3
CC(1/2)		0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	295	17% PEG3350, 100 mM Bis-Tris, pH 6.4, 200 mM ammonium nitrate, 10 mM spermine-HCl