5UW7
PCY1 Y481F Variant in Complex with Follower Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.07814 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 86.947, 59.629, 134.164 |
| Unit cell angles | 90.00, 93.12, 90.00 |
Refinement procedure
| Resolution | 86.800 - 2.370 |
| R-factor | 0.23865 |
| Rwork | 0.236 |
| R-free | 0.28405 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.324 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.900 | 2.381 |
| High resolution limit [Å] | 2.370 | 2.373 |
| Number of reflections | 55622 | 551 |
| <I/σ(I)> | 14 | 2.1 |
| Completeness [%] | 99.2 | 96.2 |
| Redundancy | 6.2 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 23% PEG 3,350, 0.1 M magnesium chloride, 0.1 M HEPES free acid (pH 7.5), 7 mg/mL protein, Protein was preincubated with 1 mM PSA1 [14-32] |
