5UW0

Activated state yGsy2p in complex with UDP-2-fluoro-2-deoxy-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Detector technology	PIXEL
Collection date	2015-03-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	193.516, 204.705, 206.514
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	145.380 - 2.730
R-factor	0.20012
Rwork	0.199
R-free	0.22449
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kqm
RMSD bond length	0.010
RMSD bond angle	1.384
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall
Low resolution limit [Å]	145.380
High resolution limit [Å]	2.730
Rmerge	0.055
Rpim	0.046
Number of reflections	102119
<I/σ(I)>	11.97
Completeness [%]	99.4
Redundancy	4.21
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	25 - 30 % Peg 300, 0.1 M Bis-Tris pH 6.1-6.4, 25 mM G-6-P, 10 mM UDP-FDG