5UVX

BRD4 Bromodomain 2 with A-1359643

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	56.135, 65.058, 72.808
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.500 - 1.530
R-factor	0.182
Rwork	0.181
R-free	0.19700
RMSD bond length	0.010
RMSD bond angle	0.920
Data reduction software	XDS (2.11.7)
Data scaling software	SCALA
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.500
High resolution limit [Å]	1.530
Rmerge	0.046
Number of reflections	41039
<I/σ(I)>	20
Completeness [%]	100.0
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	Protein Buffer : 10 mM HEPES PH 7.5 100 mM NaCl 5 mM DTT Crystallization : 15 % (v/v) Ethanol Tris PH 7.0