5UVK

Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-crystals using Synchrotron Radiation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	298
Detector technology	PIXEL
Collection date	2016-08-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03
Spacegroup name	H 3 2
Unit cell lengths	186.400, 186.400, 60.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.010 - 3.100
R-factor	0.17723
Rwork	0.172
R-free	0.20474
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ziz
RMSD bond length	0.011
RMSD bond angle	2.093
Data reduction software	CrystFEL (0.6.2)
Data scaling software	CrystFEL (0.6.2)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	3.180
High resolution limit [Å]	3.100	3.100
Number of reflections	6280
<I/σ(I)>	7.6
Completeness [%]	99.7
Redundancy	156.4
CC(1/2)	0.986	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7	277	75 mM HEPES pH 7, 20 mM MgCl2, 9 % PEG 3350