5UVE
Crystal Structure of the ABC Transporter Substrate-binding protein BAB1_0226 from Brucella abortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 |
| Unit cell lengths | 82.602, 92.738, 95.124 |
| Unit cell angles | 95.19, 110.87, 111.35 |
Refinement procedure
| Resolution | 41.783 - 2.500 |
| R-factor | 0.2282 |
| Rwork | 0.226 |
| R-free | 0.27740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ne4 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.955 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 80763 | 4014 |
| <I/σ(I)> | 9.13 | 1.63 |
| Completeness [%] | 97.8 | 97.8 |
| Redundancy | 2.2 | 2.1 |
| CC(1/2) | 0.445 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M calcium chloride, 0.1 M Tris pH 8.5, 25 %(v/w) PEG4000 |
