5UUT

N-myristoyltransferase 1 (NMT) bound to myristoyl-CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-10
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	78.778, 177.582, 58.566
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.454 - 2.252
R-factor	0.1733
Rwork	0.171
R-free	0.22110
RMSD bond length	0.008
RMSD bond angle	1.010
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.250
Number of reflections	39222
<I/σ(I)>	22.09
Completeness [%]	99.7
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	22.5% PEG 4000, 5 mM NiCl2, 100 mM sodium citrate pH 4.5, and 2.5% glycerol