5UT5
JAK2 JH2 in complex with GLPG0634
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-14 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.440, 57.381, 60.556 |
| Unit cell angles | 90.00, 110.03, 90.00 |
Refinement procedure
| Resolution | 41.751 - 1.900 |
| R-factor | 0.1618 |
| Rwork | 0.160 |
| R-free | 0.20050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fvp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.836 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.751 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 22747 | |
| <I/σ(I)> | 19.1 | 3.2058 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M Tris, pH 8.0 0.2M Sodium acetate 12-20% PEG 4,000 |
