5USN
Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssRNA/ssDNA chimera (rGrGrUTACGGT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.640, 59.800, 66.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.339 - 1.900 |
| R-factor | 0.1872 |
| Rwork | 0.183 |
| R-free | 0.22370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hik |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.104 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (0.5.25) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 59.830 | 59.830 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.082 | 0.065 | 0.273 |
| Rmeas | 0.093 | 0.075 | 0.306 |
| Rpim | 0.042 | 0.037 | 0.135 |
| Total number of observations | 61629 | ||
| Number of reflections | 13518 | 1284 | |
| <I/σ(I)> | 13.1 | ||
| Completeness [%] | 99.8 | 91.4 | 100 |
| Redundancy | 4.6 | 3.6 | 4.7 |
| CC(1/2) | 0.995 | 0.994 | 0.953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 277 | 100 mM Tris, 0.2 mM sodium formate, 15% PEG 4000 |
