5USD
Crystal structure of MccF-like protein (BA_5613) in the complex with aspartyl sulfamoyl adenylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.456, 85.808, 124.804 |
| Unit cell angles | 90.00, 92.32, 90.00 |
Refinement procedure
| Resolution | 32.611 - 2.095 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.20960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fd8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.619 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.611 |
| High resolution limit [Å] | 2.095 |
| Rmerge | 0.093 |
| Number of reflections | 84529 |
| <I/σ(I)> | 10.5 |
| Completeness [%] | 92.7 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | 0.1 M TRIS, 25% PEG 3350 |
