5URM
Crystal structure of human BRR2 in complex with T-1206548
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 103.863, 105.874, 135.285 |
| Unit cell angles | 71.14, 70.48, 89.83 |
Refinement procedure
| Resolution | 29.477 - 2.800 |
| R-factor | 0.2145 |
| Rwork | 0.212 |
| R-free | 0.25980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.155 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.500 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 123431 |
| <I/σ(I)> | 8.1 |
| Completeness [%] | 98.2 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.25-1.5M sodium malonate, 100mM sodium citrate pH 5.0-5.3, 10mg/ml BRR2 |
