5UR0
Crystallographic structure of glyceraldehyde-3-phosphate dehydrogenase from Naegleria gruberi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-25 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.45861 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.743, 94.552, 90.932 |
| Unit cell angles | 90.00, 99.96, 90.00 |
Refinement procedure
| Resolution | 29.854 - 1.940 |
| R-factor | 0.1545 |
| Rwork | 0.154 |
| R-free | 0.19840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e6a edited |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.302 |
| Data scaling software | XSCALE (VERSION May 1, 2016 BUILT=20160617) |
| Phasing software | PHASER (2.5.5) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.536 | 2.000 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.073 | 0.701 |
| Number of reflections | 102380 | |
| <I/σ(I)> | 18.26 | 2.28 |
| Completeness [%] | 99.1 | 96.4 |
| Redundancy | 6 | 4.3 |
| CC(1/2) | 0.999 | 0.787 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 291 | 2.5% (m/V) PEG 1000, 50 mM acetate buffer (pH 4.8), 25.7% (m/V) PEG 2000 MME |
