5UQ1
Crystal structure of human Cdk2-Spy1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2016-03-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.359, 75.359, 325.791 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.111 - 3.201 |
| R-factor | 0.2079 |
| Rwork | 0.205 |
| R-free | 0.26110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.049 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.111 | 3.420 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.170 | 0.800 |
| Number of reflections | 17634 | 8971 |
| <I/σ(I)> | 7.9 | 2.3 |
| Completeness [%] | 94.7 | 81.3 |
| Redundancy | 6.2 | 3.4 |
| CC(1/2) | 0.980 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 295 | 1M lithium chloride, 12% PEG 6000, and 0.1M MES pH 6.2, harvested and flash frozen in the same solution with 20% PEG 200. |
