5UPM
Crystal structure of BhGH81 mutant in complex with laminaro-triose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-09 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97971 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.520, 93.680, 159.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.770 - 1.700 |
| R-factor | 0.1473 |
| Rwork | 0.146 |
| R-free | 0.16680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t49 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.358 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.770 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.249 | |
| Rpim | 0.141 | |
| Number of reflections | 107049 | |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 91 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 15% PEG 10000, 0.2 M ammonium acetate, 0.1 M bis-tris |
