5UPJ

HIV-2 PROTEASE/U99283 COMPLEX

Experimental procedure

Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1993-02-23
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	33.382, 45.281, 133.359
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.189 *
Rwork	0.182
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	EARLIER STRUCTURE FROM SAME LABORATORY
RMSD bond length	0.016
RMSD bond angle	2.547
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	MERLOT
Refinement software	CEDAR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.064	0.303
Total number of observations	33887 *
Number of reflections	8579
<I/σ(I)>	13.4	3.55
Completeness [%]	90.0	73
Redundancy	4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *		CRYSTALS WERE GROWN AT ROOM TEMPERATURE IN 4-7 UL HANGING DROPS OF EQUAL VOLUMES OF PROTEIN AT 8-10 MG/L AND WELL SOLUTION OF 30-35%(W/V) PEG 4000 IN 0.1 M HEPES AND PH RANGE 6.8 - 7.6., pH 7.1, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8-10 (mg/ml)
2	1	reservoir	PEG4000	30-35 (%(w/v))
3	1	reservoir	HEPES	0.1 (M)
4	1	reservoir	n-butanol	1-3 (%)