5UP7

Crystal Structure of the Ni-bound Human Heavy-Chain Ferritin 122H-delta C-star variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	F 4 3 2
Unit cell lengths	180.150, 180.150, 180.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.005 - 1.790
R-factor	0.1606
Rwork	0.159
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cmr
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.005
High resolution limit [Å]	1.790	1.790
Number of reflections	24176
<I/σ(I)>	12.5
Completeness [%]	100.0
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	Reservoir: 500 uL total volume: 25 mM Tris (pH 8), 10 mM CaCl2, 1 mM NiCl2, 1% PEG 6000 Sitting Drop: 2 uL reservoir, 2 uL of 12.5 uM ferritin