5UNE
Dimerized Structure Gives Further Insight Into the Function of the Novel RNA Gene: HAR1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2009-05-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.8550 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.562, 39.826, 80.974 |
| Unit cell angles | 90.00, 111.83, 90.00 |
Refinement procedure
| Resolution | 39.035 - 2.900 |
| R-factor | 0.205628872665 |
| Rwork | 0.201 |
| R-free | 0.24942 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.183 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 75.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.042 |
| Rpim | 0.086 |
| Number of reflections | 8346 |
| <I/σ(I)> | 7.4 |
| Completeness [%] | 99.9 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 500mM Tris-HCl, 1MKCl, 50mM MgCl2 |
