5UND
Crystal Structure of CTCF(ZnF 4-10) With 28-mer DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-23 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.072, 73.825, 93.289 |
| Unit cell angles | 90.00, 91.38, 90.00 |
Refinement procedure
| Resolution | 34.613 - 2.549 |
| R-factor | 0.2124 |
| Rwork | 0.211 |
| R-free | 0.23670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kkq |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.440 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.600 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.101 | 0.986 |
| Rpim | 0.044 | 0.458 |
| Number of reflections | 33435 | 3301 |
| <I/σ(I)> | 14.9 | 1.4 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 6.3 | 5.4 |
| CC(1/2) | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 28% PEG 2000 MME 100mM Bis-Tris, pH 6.5 |
