5UMS

Crystal structure of middle double PH domain of human FACT complex subunit SSRP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-10-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	63.810, 82.290, 48.505
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.157 - 1.569
R-factor	0.1985
Rwork	0.198
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gcl
RMSD bond length	0.004
RMSD bond angle	0.623
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_972)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.160
High resolution limit [Å]	1.569
Number of reflections	35760
<I/σ(I)>	29.6
Completeness [%]	93.1
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	100 mM Bis-Tris, pH 6.5, 28% polyethylene glycol monomethyl ether 2000