5UMI
Clostridium difficile TcdA-CROPs bound to PA50 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-08 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 93.685, 77.330, 97.781 |
Unit cell angles | 90.00, 90.91, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.884 | 3.230 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.142 | 0.640 |
Number of reflections | 21838 | |
<I/σ(I)> | 8.5 | |
Completeness [%] | 96.3 | 83.6 |
Redundancy | 6.3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.1 M Tris pH 7.5 0.6 M calcium chloride 19% PEG 3350 |