5UM6

Crystal Structure of S. pombe Uba1 in a closed conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	108
Detector technology	CCD
Collection date	2015-07-20
Detector	RAYONIX MX300-HS
Wavelength(s)	1.08
Spacegroup name	P 21 3
Unit cell lengths	145.102, 145.102, 145.102
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.244 - 2.794
R-factor	0.2075
Rwork	0.206
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ii3
RMSD bond length	0.003
RMSD bond angle	0.508
Data reduction software	HKL-2000 (v705b)
Data scaling software	HKL-2000 (v705b)
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.794	2.790
Rmerge	0.066	0.726
Rpim	0.200
Number of reflections	25520
<I/σ(I)>	39.2
Completeness [%]	100.0	100
Redundancy	12.3	12.5
CC(1/2)		0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	0.1M Sodium Citrate pH5.6-6.0 1.0-1.2 Ammonium Sulfate