5ULW

Structure of human DNA polymerase iota bound to template 1-methyl-deoxyadenosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-26
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 65 2 2
Unit cell lengths	97.918, 97.918, 202.473
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.959 - 2.617
R-factor	0.2074
Rwork	0.204
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fll
RMSD bond length	0.008
RMSD bond angle	1.008
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.710
High resolution limit [Å]	2.620	2.620
Number of reflections	18059
<I/σ(I)>	27.9
Completeness [%]	99.7	69.5
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	50 mM MES, pH 6.0, 18 % PEG 5KMME, 0.4 M Ammonium Sulfate