5ULM

Structure of the ASK1 central regulatory region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	74.234, 57.117, 103.575
Unit cell angles	90.00, 104.87, 90.00

Refinement procedure

Resolution	47.100 - 2.100
R-factor	0.23499
Rwork	0.233
R-free	0.26716
Structure solution method	SIRAS
RMSD bond length	0.012
RMSD bond angle	1.435
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.100
High resolution limit [Å]	2.100
Number of reflections	49197
<I/σ(I)>	8.3
Completeness [%]	99.4
Redundancy	5
CC(1/2)	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	290	0.1 M Sodium Fluoride, 10 % PEG 3350,