5UL6
The molecular mechanisms by which NS1 of the 1918 Spanish influenza A virus hijack host protein-protein interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-10-25 |
| Detector | RIGAKU |
| Wavelength(s) | 1.45 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.650, 39.260, 29.030 |
| Unit cell angles | 90.00, 98.43, 90.00 |
Refinement procedure
| Resolution | 27.351 - 1.450 |
| R-factor | 0.1681 |
| Rwork | 0.167 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ih2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.804 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.351 |
| High resolution limit [Å] | 1.450 |
| Number of reflections | 10105 |
| <I/σ(I)> | 9 |
| Completeness [%] | 91.9 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 278 | 0.2 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH4.6, 30% w/v Polyethylene glycol monomethyl ether 2000 |
